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目的：建立血浆中 Ｂ Ｚ类失能剂的高效液相二极管矩阵检测分析方法，为 Ｂ Ｚ类失能剂中毒病人的临床诊断提供分析手段。方法：采用 Ｓｅｐ Ｐａｋ Ｃ１８ 柱固相提取，乙酸乙酯洗脱， Ｒｅｓｏｌｖｅ Ｃ１８ 反相柱分离，０．１２％ 三氟乙酸（ Ｔ Ｆ Ａ）的 ５０％ 甲醇溶液梯度洗脱，二极管矩阵检测器（ Ｄ Ａ Ｄ） 三波长（２２０±４）ｎｍ ，（２５４±４）ｎｍ ，（３００±４）ｎｍ 检测。结果：血浆中 Ｈ Ｐ Ｌ Ｃ Ｄ Ａ Ｄ 法检测 ４ 种失能剂的线性范围为 ０．１～１．２ μｇ，回收率为６２．７６％ ～９２．２０％ ，标准偏差为 ３．４６％ ～１１．８４％ ，最低检出限为 １００～４００ ｎｇ。流动相中 Ｔ Ｆ Ａ 和甲醇浓度及梯度洗脱是影响分离效果和色谱峰形的主要因素。结论：本文为临床诊断提供简便、快速、灵敏的检测方法，血浆蛋白不干扰测定。４ 种失能剂在血浆中采用 Ｈ Ｐ Ｌ Ｃ Ｄ Ａ Ｄ 法同时分离测定目前未见报道。
OBJECTIVE: To establish a HPLC matrix detection and analysis method for plasma BZ-type incapacitating agents, and to provide an analytical method for the clinical diagnosis of BZ-type incapable toxic patients. Methods: The solid phase extraction was performed on a Sep-Pak C18 column, eluted with ethyl acetate, separated on a Resolve C18 reverse phase column and eluted with a gradient of 0.12% trifluoroacetic acid (TFA) in 50% methanol. The diode matrix detector (D A D) three wavelengths (220 ± 4) nm, (254 ± 4) nm, (300 ± 4) nm detection. Results: The linear range of the four kinds of incapacitating agents detected by HPCL-D A D was 0.1-1.2 μg, the recovery was 62.76% -92.20%, and the standard deviation was 3. 46% ~ 11.84%, the lowest detection limit was 100 ~ 400 ng. The mobile phase of T F A and methanol concentration and gradient elution is the main factor affecting the separation and peak shape. Conclusion: This article provides a simple, rapid and sensitive detection method for clinical diagnosis, plasma protein does not interfere with the determination. Four kinds of incapacitating agents in the plasma using H P L C D A D simultaneous determination of the method has not been reported.