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目的 探讨不同DNA载体及靶基因对DNA疫苗免疫效果的影响。 方法 用已构建的不同载体HBV S基因疫苗(pCR3.1-S,pcDNA3-S)和HBVC基因疫苗(pCR3.1-C)分别给Balb/c小鼠多点肌肉注射,2wk后追加免疫一次,用ELISA法及MTT法分别检测小鼠血清抗-HBs及抗HBc及脾细胞对HBsAg或HBcAg的特异性增殖反应。 结果 免疫接种2wk后小鼠血清抗-HBs及抗HBc抗体均明显高于对照组,载体pCR3.1的表达效力稍高于pcDNA3,但同一基因不同载体间无显著差异,不同靶基因血清抗体滴度及脾细胞对HBsAg或HBcAg的刺激指数均明显高于对照组,刺激指数pCR3.1-C组明显高于单纯pCR3.1-S注射组。 结论 两种载体均可以诱导较强的体液免疫应答;但同一基因不同载体间无显著差异,HBVS和C基因疫苗均可诱导较强的体液和细胞免疫应答强度;C基因以细胞免疫增高为主。
Objective To investigate the effect of different DNA vectors and target genes on DNA vaccine immunization. Methods Balb / c mice were intramuscularly injected with the constructed HBV S gene vaccine (pCR3.1-S, pcDNA3-S) and HBVC gene vaccine (pCR3.1-C), respectively, The specific proliferative responses of anti-HBs and anti-HBc and splenocytes of mice to HBsAg or HBcAg were detected by ELISA and MTT respectively. Results Serum anti-HBs and anti-HBc antibodies of mice immunized 2wk after vaccination were significantly higher than that of the control group, the expression efficiency of pCR3.1 was slightly higher than that of pcDNA3, but there was no significant difference between the different vectors of the same gene. Degree and spleen cells to HBsAg or HBcAg stimulation index were significantly higher than the control group, stimulation index pCR3.1-C group was significantly higher than the simple pCR3.1-S injection group. Conclusion Both vectors can induce a strong humoral immune response. However, there is no significant difference between different vectors of the same gene. HBV and C gene vaccines can induce strong humoral and cellular immune responses. C gene is mainly based on cellular immunity .