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目的研究福州市乙肝病毒基因型和亚型分布及其与T1762/A1764、A1896变异的关系,为完善预防、诊断、治疗病毒感染的策略和方法提供科学依据。方法应用型特异性引物PCR法检测HBsAg阳性血清的基因型,应用PCR-RFLP方法检测基因亚型、T1762/A1764变异和A1896变异。结果282份HBsAg阳性血清样品中103份未能成功分型,其余179份样品中B基因型122份(68.2%),C基因型54份(30.2%),B+C型3份(1.7%),未检测到其他基因型。随机选取的100份B基因型样品中,Ba亚型71份(71.0%),Bj亚型8份(8.0%),未能分亚型者21份(21.0%)。54份C基因型样品中Ce亚型31份(57.4%),Cs亚型14份(25.9%),Ce+Cs1份(1.9%),未能分亚型者8份(14.8%)。T1762/A1764变异标本9份(8.7%),Ce亚型变异率最高(29.2%),Ba亚型次之(3.3%),Cs和Bj亚型未检测到变异株,T1762/A1764变异在不同基因型和亚型间的分布差异有统计学意义(P<0.05)。A1896变异标本10份(10.0%),Ba亚型变异率最高(14.0%),Cs亚型次之(10.0%),Ce亚型最低(4.0%),不同基因型和亚型中的变异差异无统计学意义(P>0.05)。HBeAg阳性和阴性样品中的基因型和亚型分布差异无统计学意义(P>0.05)。结论福州市乙肝病毒以B、C基因型为主,Ba、Ce亚型占优势,HBV各基因型和亚型发生T1762/A1764、A1896变异的模式不同。
Objective To study the distribution of genotypes and subtypes of hepatitis B virus in Fuzhou and its relationship with the variations of T1762 / A1764 and A1896, so as to provide scientific basis for perfecting the strategies and methods of preventing, diagnosing and treating viral infections. Methods Genotypes of HBsAg positive serum were detected by PCR and RFLP. The genotypes of T1762 / A1764 and A1896 were detected by PCR-RFLP. RESULTS: Of the 282 samples with HBsAg positive serum, 103 were unsuccessful, and the other 129 samples contained 122 (68.2%) B genotypes, 54 (30.2%) C genotypes and 3 (1.7% ), No other genotypes were detected. Of the 100 randomly selected B genotypes, 71 were Ba subtypes (71.0%), 8 (8.0%) were Bj subtypes and 21 (21.0%) were subtypes. Among 54 C genotype samples, 31 (57.4%) were Ce subtype, 14 (25.9%) were Cs subtype, 1 (1.9%) were Ce + Cs and 8 (14.8%) were subtype subtype. There were 9 (8.7%) T1762 / A1764 variant samples, the highest mutation rate of Ce subtype (29.2%), the second (3.3%) Ba subtype, and no variation of T1762 / A1764 in Cs and Bj subtypes The distribution of genotypes and subtypes had statistical significance (P <0.05). The mutation rate of Ba subtype was the highest (14.0%), that of Cs subtype (10.0%) was the lowest (4.0%), and that of the other genotypes and subtypes was 10 (10.0% No statistical significance (P> 0.05). There was no significant difference in the distribution of genotypes and subtypes in HBeAg positive and negative samples (P> 0.05). Conclusion The genotypes B and C are dominant in Hepatitis B virus in Fuzhou, while the genotypes Ba and Ce are predominant. The patterns of T1762 / A1764 and A1896 variation in HBV genotypes and subtypes are different.