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目的 确立血脾屏障的组成及形态构筑 ,探讨血脾屏障在脾功能中的作用 .方法 采用健康昆明株小白鼠 10 0只 ,随机分成 10组经尾静脉注射 5 %墨汁混悬液 (0 .2～ 0 .3m L /只 ) ,分别在 1,3,6 ,12 h及 1,3,5 ,7,14,2 8d取脾进行组织学、免疫组化第 因子相关抗原染色 ,在光镜、电镜下观察 ,同时检测内皮细胞、巨噬细胞吞噬墨汁颗粒的数量 .结果 脾血窦内皮细胞、巨噬细胞对第 因子相关抗原呈阳性反应 .注射墨汁混悬液后 3h,6 d内皮细胞、巨噬细胞内可见墨汁颗粒 ,在 2 4h,3d组最多 ,随后逐渐减少 ,墨汁颗粒主要集中在红髓及边缘区 .结论 血脾屏障由脾窦内皮细胞、基底膜、巨噬细胞组成 ,位于红髓及边缘区
Objective To establish the composition and morphology of the blood-spleen barrier and explore the role of the blood-spleen barrier in the spleen function.Methods 10 healthy mice were randomly divided into 10 groups and injected with 5% ink suspension through tail vein. 2 ~ 0. 3m L / only). The spleens were harvested at 1, 3, 6, 12 and 1, 3, 5, 7, 14 and 28 days respectively for histological and immunohistochemical staining of factor- And the numbers of phagocytic ink particles of endothelial cells and macrophages were detected at the same time.Results The positive reaction of spleen blood sinus endothelial cells and macrophages to factor-related antigen.After injecting the ink suspension for 3h and 6d, The ink particles were visible in the cells and macrophages in 24 h and 3 d groups, then decreased gradually, and the ink particles mainly concentrated in the red pulp and marginal zone.Conclusion The blood-spleen barrier consisted of splenic sinus endothelial cells, basal membrane and macrophages , Located in the red pulp and marginal zone