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目的了解2009-2011年广州市手足口病病例标本中病原学分类情况。方法采集临床诊断为手足口病患者的粪便、咽拭子及肛拭子标本,用荧光定量PCR法同时检测总肠道病毒、CoxA16和EV71。结果广州市手足口病的流行每年有1个高峰和1个次高峰。第1个高峰4～8月的病原体为CoxA16、EV71和其他肠道病毒同时存在,次高峰为11月～次年1月,病原体主要为CoxA16。结论 CoxA16、EV71和其他肠道病毒为广州市近年手足口病的主要病原。
Objective To understand the etiological classification of hand, foot and mouth disease in Guangzhou from 2009 to 2011. Methods Stool samples, throat swabs and anal swab samples from patients with hand-foot-mouth disease were collected. Total enterovirus, CoxA16 and EV71 were detected by real-time PCR. Results The prevalence of hand, foot and mouth disease in Guangzhou has one peak and one sub-peak every year. The first peak of April to August pathogens CoxA16, EV71 and other enterovirus coexist, the next peak from November to January the following year, the main pathogen is CoxA16. Conclusion CoxA16, EV71 and other enteroviruses are the major pathogens of HFMD in Guangzhou in recent years.