论文部分内容阅读
目的 在豚鼠肠系膜下神经节 (IMG)细胞上观察不同 5 羟色胺 (5 HT)受体亚型拮抗剂对非胆碱能迟慢兴奋性突触后电位 (LS EPSP)的作用。方法 应用离体细胞内记录技术。结果 赛庚啶 (cyproheptadine ,5 HT1/ 2 受体拮抗剂 )及BRL 2 492 4(5 HT1P受体拮抗剂 )可逆的阻抑 5 HT敏感细胞的LS EPSP ,而mianserin(5 HT2 受体拮抗剂 ) ,MDL72 2 2 2 (5 HT3 受体拮抗剂 ) ,spiperone(5 HT1A受体拮抗剂 )对LS EPSP无显著影响 ;用MCPP(5 HT1P受体激动剂 )持续灌流IMG使 5 HT敏感细胞脱敏后可逆的阻抑LS EPSP的发生。结论 5 HT敏感细胞的LS EPSP是由 5 HT1P受体介导的。
Objective To observe the effect of different 5-HT receptor subtype antagonists on chronic cholinergic excitatory postsynaptic potential (LS EPSP) in guinea pig lower mesenteric ganglion (IMG) cells. Methods Using in vitro intracellular recording technique. Results Cyproheptadine (5 HT1 / 2 receptor antagonist) and BRL 2 492 4 (5 HT1P receptor antagonist) reversibly suppressed LS EPSP in 5 HT-sensitive cells, whereas mianserin (5 HT2 receptor antagonist ), MDL72 2 2 2 (5 HT3 receptor antagonist), and spiperone (5 HT1A receptor antagonist) had no significant effect on LS EPSP. Continuous perfusion of IMG with MCPP (5 HT1P receptor agonist) Sensitive and reversible suppression of the occurrence of LS EPSP. CONCLUSIONS: LS-EPSP in 5 HT-sensitive cells is mediated by the 5 HT1P receptor.