论文部分内容阅读
目的：研究精加压素片段（４－８）在大鼠海马中的信号跨膜转导．方法：比较大鼠海马切片在药物刺激后的ＭＡＰＫ活性（ＭＫ）和ＣａＭＫⅡ自身磷酸化水平（ＫⅡ）的变化．结果：（４－８）的拮抗剂ＺＤＣ（Ｃ）ＰＲ及ＧＰＣＲ的抑制剂ＰＴＸ分别都能阻断（４－８）引起的（ＭＫ）和（ＫⅡ）的增高，但都不影响ＡＶＰ诱导的（ＭＫ）变化；ＰＭＢ抑制（４－８）诱导的（ＭＫ）增高而对（ＫⅡ）无影响；ＴＰＡ单独可以刺激（ＭＫ）增高达（４－８）的（ＭＫ）水平，同时使（ＫⅡ）停留在对照水平；（ＭＫ）的增高不被ＫＮ６２阻断；与ＡＶＰ不同，（４－８）不影响ｃＡＭＰ水平．结论：精加压素片段（４－８）通过未知ＧＰＣＲ和Ｇ０介导一分支信号途径．
AIM: To investigate the signal transmembrane transduction of serovar hormones (4-8) in rat hippocampus. Methods: The changes of MAPK activity (MK) and autophosphorylation of CaMKII (KII) in hippocampal slices of rats were compared. RESULTS: ZDC (C) PR (4-8) and PTX (inhibitor of GPCR) blocked (4-8) -induced (MK) and (KⅡ) (MK); PMB inhibited (4-8) -induced (MK) increased but had no effect on (KⅡ); TPA alone increased MK up to (4-8) (MK) ) Stayed at the control level; (MK) was not blocked by KN-62; unlike AVP, (4-8) did not affect cAMP levels. Conclusion: The vasopressin fragments (4-8) mediate a branched signaling pathway through unknown GPCRs and G0.