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目的 构建原核分泌型表达载体并高效表达胰岛素样生长因子I(IGF I)。方法 构建出 2种分泌型表达载体 ,命名为pCSA和pCST ,并将胰岛素样生长因子I(IGF I)基因插入pCSA和pCST中 ,转化E .coliW3110 ,经筛选获得工程菌pCSA/IGF I/W3110和pCST/IGF 1/W3110 ,并进行表达。结果 经SDS PAGE检测 ,在相对分子质量 76 0 0处有明显表达带 ,Westernblot表明重组蛋白具有IGF I的抗原性。结论 原核分泌型IGF I的高效表达 ,为进一步研究人IGF I的功能和生物学特性打下基础
Objective To construct prokaryotic expression vector and express insulin - like growth factor I (IGF I) efficiently. Methods Two secreting expression vectors were constructed and named as pCSA and pCST. The IGF I gene was inserted into pCSA and pCST and transformed into E. coli W3110. The pCSA / IGF I / W3110 And pCST / IGF 1 / W3110, and expressed. The results of SDS PAGE showed that there was a significant expression of the molecular weight of 76 0 0, Western blot showed that the recombinant protein has IGF I antigenicity. Conclusion The high expression of prokaryotic secretory IGF I can lay a foundation for further study on the function and biological characteristics of human IGF I