论文部分内容阅读
从日本海岸普遍生长的海洋绿藻孔石莼(Ulvapertusa)中提到一种具负性肌力作用的腺苷,这在海洋生物中还是首次发现。提取分离步骤:新鲜孔石莼5.6kg 的丙酮提取液经减压浓缩后,以正己烷和乙酸乙酯洗脱,浓缩所得棕色粉末以甲醇提取,滤液浓缩得一浅棕色油状物。进一步用TSK-Gel G 300 S~2凝胶柱(220ml)纯化。收集洗脱水流份得[(1)～(6);水-甲醇(80:20)得流份(7)、
A negative inotropic adenosine is mentioned in the marine algae Ulvapertusa, a species commonly found on the coast of Japan and was first found in marine life. Extraction and separation steps: 5.6kg of fresh hole stone 莼 acetone extract was concentrated under reduced pressure, with n-hexane and ethyl acetate eluate, the resulting brown powder was concentrated to methanol extraction, the filtrate was concentrated to a light brown oil. Further purification was performed on a TSK-Gel G 300 S-2 gel column (220 ml). Collect fractions of elution water [(1) ~ (6); water - methanol (80:20)