论文部分内容阅读
细胞色素C位于需氧细胞线粒体内膜面,是一种重要的电子载体,心脏内其浓度尤高.它能将电子传递给线粒体内的分子氧,使之在氧化磷酸化过程中产生ATP.本文旨在验证细胞色素C能否在急性冠状动脉闭塞时保护缺血心肌,并研究其对低灌注节段心肌功能的影响.体重22～31kg的36条狗以戊巴比妥(20mg/kg)静脉麻醉下,气管内插管,采用容量呼吸器以室内空气进行换气.左颈动脉和静脉内插入聚乙烯管,分别监测动脉压和用于给药.经左第5肋间开胸将心脏悬吊在心包支架内.在右第5肋间开胸将超声心动图的换能器置于右室游离壁上.尔后在第1对角支近端分离左前降支(LAD),并以一丝线缝闭之.在LAD闭塞后1分钟,左房内注入2×10~?
Cytochrome C is an important electron carrier located in the mitochondrial inner membrane surface of aerobic cells and its concentration in the heart is very high, which can transfer electrons to molecular oxygen in the mitochondria to generate ATP during oxidative phosphorylation. This article aims to verify whether cytochrome C can protect ischemic myocardium during acute coronary occlusion and to investigate its effect on myocardial function in hypoperfusion perfusion.Among 36 dogs weighing 22-31 kg, pentobarbital (20 mg / kg ) Under intravenous anesthesia, endotracheal intubation, the use of volume respirator to ventilate the indoor air. Left carotid artery and vein inserted polyethylene tube, respectively, for monitoring arterial pressure and for administration .Through the left fifth intercostal thoracotomy The heart was suspended in a pericardial stent and the echocardiographic transducer was placed on the right ventricular free wall at the right intercostal 5. The left anterior descending (LAD) was then separated proximal to the first diagonal branch, And closed with a trace of suture.After LAD occlusion 1 minute, left atrial injection of 2 × 10 ~?