目的:验证同种异体移植瓣膜(VA)钙化起因于有骨调节蛋白表达的骨化过程的假说。方法:对被移植用于治疗心脏瓣膜功能障碍的15例VA进行研究。将VA与正常主动脉瓣膜(n=20)进行分析比较。结果:所有VA(5例主动脉瓣、10例肺动脉瓣)出现重度钙化和纤维化。免疫组织化学研究显示基质细胞表达骨特异性转录因子Cbfa-1。钙化区域有骨碱性磷酸酶表达。VA的平滑肌(α-SM)肌动蛋白免疫染色多于正常瓣膜,其中有6例VA瓣膜在钙化性结节附近形成细胞簇。 OBJECTIVE: To verify the hypothesis that atorification of allograft valve (VA) calcifications results from the ossification process with osteomodulin expression. Methods: 15 VA patients transplanted for the treatment of cardiac valve dysfunction were studied. VA was compared with normal aortic valves (n = 20). Results: All VA (5 aortic valves, 10 pulmonary valves) developed severe calcification and fibrosis. Immunohistochemical studies showed that stromal cells express the bone-specific transcription factor Cbfa-1. Calcified area with bone alkaline phosphatase expression. VA smooth muscle (α-SM) actin immunostains more than normal valves, of which 6 VA valves form clusters of cells near calcified nodules.