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目的:首次调查福建省五个地区乙型肝炎病毒(HBV)基因型的分布状况,并探讨HBV基因型与HBV,相关肝病临床的可能相关性。方法:收集福州市、厦门市、泉州市、三明市、莆田市等地区慢性HBV感染者的血清,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)法检测HBV基因型,应用多分类logistic回归分析、对应分析研究HBV基因型与临床的相关性。结果:431份HBV DNA阳性的血清中基因B型275例(63.8％),C型100例(23.2％),D型及其混合型共51例(11.8％)。未见A、E、F型。多分类logistic回归分析显示泉州和三明地区HBV基因B型所占比例显著高于福州地区(P=0.002;P=0.006);无症状携带者、慢性肝炎、重型肝炎都是以基因B型为主要基因型;基因C型在肝硬化中所占比例(47％)显著高于无症状携带者(14.5％)和重型肝炎组(14.7％)(P=0.009,P<0.001);基因B型的e抗原阳性率(52.4％)显著低于C型(56％)(P=0.008);基因D型患者e抗原阳性率(30.8％)也低于C型(P=0.051)。对应分析表明HCC与基因D型及其混合型关系密切。结论:①福建省HBV感染以基因B型为主,其次是C型,也存在基因D型的流行。②福建省部分地区基因型B和C的分布可能存在差异。③基因B型在年轻患者中可能与重型肝炎的发展有关;基因C型在年长患者中可能更易导致肝硬化。④基因D型与
Objective: To investigate the distribution of hepatitis B virus (HBV) genotypes in five regions of Fujian Province for the first time and to explore the possible relationship between HBV genotypes and HBV and related liver diseases. Methods: The serum of patients with chronic HBV infection in Fuzhou, Xiamen, Quanzhou, Sanming, Putian and other regions were collected. The HBV genotypes were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) Multi-classification logistic regression analysis was used to analyze the association between HBV genotypes and clinical features. Results: Of 431 HBV DNA positive sera, 275 (63.8%) were genotype B, 100 (23.2%) were C and 51 (11.8%) were D and mixed. No A, E, F type Multivariate logistic regression analysis showed that the proportion of HBV genotype B in Quanzhou and Sanming was significantly higher than that in Fuzhou (P = 0.002; P = 0.006); asymptomatic carriers, chronic hepatitis and severe hepatitis were mainly genotype B Genotype; genotype C was significantly higher in patients with cirrhosis (47%) than in asymptomatic carriers (14.5%) and severe hepatitis (14.7%) (P = 0.009, P <0.001) The positive rate of e antigen (52.4%) was significantly lower than that of C (56%) (P = 0.008). The positive rate of e antigen in gene D was also lower than that in C (P = 0.051). Corresponding analysis showed that HCC is closely related to genotype D and its mixed type. Conclusion: HBV infection in Fujian Province is mainly genotype B, followed by genotype C, and genotype D is also present. ② There may be differences in the distribution of genotypes B and C in some areas of Fujian Province. ③ genotype B in young patients may be related to the development of severe hepatitis; genotype C in older patients may be more likely to lead to cirrhosis. ④ gene D-type and