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N-甲基-N-亚硝基脲(MNU)和其它多种抗癌药物一样,可以引起诸如大鼠、小鼠、地鼠、猴等许多动物的消化道、神经系统、呼吸道等部位的肿瘤。近年来,短期生物学试验表明,许多致癌物对细胞有致突变作用,或引起染色体损伤。本文简要报告MNU诱导V_(79)细胞的姊妹染色单体交换(SCE)及6-巯基鸟嘌呤耐受性(6-TG)突变,并比较这两种试验的敏感性。材料与方法材料 V_(79)细胞来自里昂国际癌症研究所。MNU由西德Ivankovic实验室赠送。
N-methyl-N-nitrosourea (MNU), like many other anticancer drugs, can cause the digestive tract, nervous system, respiratory tract, etc. in many animals such as rats, mice, hamsters, and monkeys. Tumors. In recent years, short-term biological tests have shown that many carcinogens have mutagenic effects on cells or cause chromosome damage. This article briefly reports the MNU-induced sister chromatid exchange (SCE) and 6-thioguanine tolerance (6-TG) mutations in V_(79) cells and compares the sensitivity of these two assays. Materials and Methods Materials V_(79) cells were obtained from Lyon International Cancer Institute. MNU was donated by the Ivankovic laboratory in West Germany.