本文目的是了解人类单核细胞衍生的巨噬细胞(Mφ)对体外培养的恶性疟原虫的杀伤力。作者按Cottrell的方法制备单核细胞。取健康未经免疫的人静脉血50～100ml,去纤维蛋白后,将血置葡聚糖500,沉淀红细胞(RBC),然后置Nycodenz单核细胞分离培养基中。在界面上有活力的细胞达100％,并有95％的细胞具单核细胞/Mφ的形态,非特异性酯酶呈阳性。将细胞冲洗2次,悬浮于寄生虫完全培养基(PCM)中,其成分包括RPMI-1640,辅以0.25％V/VNaHCO_3,25mmol/L Hepes,50μg/ml庆大霉素,4mmol/L谷酰胺以及10％灭活A型人血清,pH7.3。单核细胞(5×10~5/ml)于疏水的Teflon袋或培养血中,37℃,5％CO_2条件下培养。3～6天后 The purpose of this paper is to understand the lethality of human monocyte-derived macrophages (Mφ) against P. falciparum cultured in vitro. The authors prepared monocytes by the method of Cottrell. Take healthy unimmunized human venous blood 50 ~ 100ml, to fibrin, the blood Glucan 500, precipitated erythrocytes (RBC), and then placed Nycodenz monocyte isolation medium. There are 100% viable cells in the interface, and 95% of the cells have monocyte / Mφ morphology with non-specific esterase positive. The cells were washed twice and resuspended in complete parasite media (PCM) and consisted of RPMI-1640 supplemented with 0.25% V / VNaHCO 3, 25 mmol / L Hepes, 50 μg / ml gentamycin, 4 mmol / Amide and 10% inactivated human type A serum, pH7.3. Monocytes (5 × 10 ~ 5 / ml) were cultured in hydrophobic Teflon bags or cultured blood at 37 ℃ under 5% CO 2. 3 to 6 days later