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从志贺氏I型痢疾杆菌LPS中分离纯化出O SP半抗原 ,以ADH为连接剂将其与TT结合形成O SP TT结合疫苗 ,并用此结合疫苗免疫NIH小鼠 ,结果显示单独使用O SP免疫后 ,小鼠血清中没有抗LPS抗体产生 ,而用O SP TT免疫后小鼠血清中产生了抗LPSIgG和IgM抗体 ,且IgG抗体水平高于IgM抗体 ;O SP TT免疫组第二次和第三次免疫后IgG抗体水平均有显著的升高 (P <0 0 1) ,但第二次和第三次免疫后血清IgM抗体虽有升高 ,但与前一次免疫相比均无显著差异 (P >0 0 5 ) ,表明O SP TT结合疫苗具有加强免疫应答效应。补体介导的体外杀菌活性试验结果证明 ,O SP TT免疫血清在 1∶16 0倍稀释后仍对志贺氏I型痢疾杆菌具有特异性杀菌活性。
Isolation and purification of O SP hapten from Shigella dysenteriae LPS and its binding to TT with ADH as an O SP TT conjugate vaccine and immunization of NIH mice with this conjugate vaccine showed that O SP After immunization, there was no anti-LPS antibody in mouse sera, but anti-LPS IgG and IgM antibodies were produced in sera of mice after immunization with O SP TT, and the level of IgG antibody was higher than that of IgM antibody. The second and After the third immunization, the level of IgG antibody increased significantly (P <0.01), but the level of serum IgM antibody increased after the second and third immunization, but no significant difference compared with the previous immunization (P> 0.05), indicating that the O SP TT conjugate vaccine has an enhanced immune response effect. Complement-mediated in vitro bactericidal activity test results show that O SP TT immune sera still have specific bactericidal activity against Shigella dysenteriae Shigella at 1:16 0-fold dilution.