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目的:探讨消炎痛型胃溃疡寒、热证模型的形成机制及寒、热方剂对该模型的作用及其方证相应的客观规律。方法:Wistar大鼠140只随机分成空白组、病模型组、病证结合模型组、证模型组。采用寒(冰水、氢氧化钠NaOH)、热(8%干辣椒粉的乙醇混悬液)因素,连续ig 7 d,建立胃寒、热证模型。随后给予寒性方剂(大黄黄连泻心汤,含生药1.8 g.kg-1)、热性方剂(理中丸,含生药3.6 g.kg-1)ig,连续4 d。末次给药后禁食30 h,ip消炎痛,建立消炎痛型胃溃疡寒、热证之“病证结合”动物模型。采用放射免疫分析法、酶联免疫法分别检测受试大鼠血清胃泌素(GAS)、胃动素(MOT)的含量,比较寒、热方剂对其含量的影响。结果:各模型组血清GAS含量均显著升高,与空白组比较有显著性差异(P<0.05或P<0.01)。与空白组比较,单消组、寒消组、热消组、单热组MOT含量明显升高(P<0.01),且热因素组升高显著。大黄黄连泻心汤、理中丸可分别显著降低热模型组、寒模型组GAS,MOT含量(P<0.05或P<0.01)。结论:通过“方证相应”理论验证了“病证结合”胃溃疡寒、热证模型的可行性;大黄黄连泻心汤对热性胃溃疡、理中丸对寒性胃溃疡的治疗作用,可通过降低血清GAS,MOT的含量而实现。
Objective: To investigate the mechanism of cold and heat syndrome of indomethacin-induced gastric ulcer and the effects of cold and heat prescriptions on the model and the corresponding objective laws of prescription. Methods: One hundred and forty Wistar rats were randomly divided into blank group, disease model group, combination of disease and syndrome model group, syndrome model group. Using cold (ice water, sodium hydroxide NaOH), hot (8% dry chili powder ethanol suspension) factors, continuous ig 7 d, the establishment of cold stomach, heat syndrome model. Subsequently given cold prescription (rhubarb Huang Xiexie Tang, containing crude drug 1.8 g.kg-1), heat prescription (Li pills, with crude drug 3.6 g.kg-1) ig, for 4 days. After the last administration of fasting 30 h, ip indomethacin, the establishment of indomethacin-induced gastric ulcer cold, heat syndrome “disease syndrome combination ” animal model. The contents of serum gastrin (GAS) and motilin (MOT) in rats were detected by radioimmunoassay and enzyme-linked immunosorbent assay (ELISA), respectively. Results: Serum GAS levels in each model group were significantly increased compared with the blank group (P <0.05 or P <0.01). Compared with the blank group, the content of MOT of Danxue group, Hanxiao group, heat-elimination group and single-heat group was significantly increased (P <0.01), and the heat-induced factor group increased significantly. Rhubarb Huanglianxinyin Decoction and Rizhong Pills could significantly reduce the levels of GAS and MOT in the heat model group and the cold model group (P <0.05 or P <0.01). Conclusions: The feasibility of “combination of disease with syndrome” and “cold and heat syndrome model of gastric ulcer” was validated by the theory of “Correspondence of square syndrome”. Rheum huanglianxiexin decoction was effective in treating gastric ulcer, Therapeutic effect can be achieved by reducing serum GAS, MOT content.