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球孢白僵菌是一种最常见的虫生真菌,已被广泛应用于害虫生物防治。利用SSR简单序列重复(simple sequence repeat,SSR)分子标记对来自安徽省麻姑山马尾松林的102株球孢白僵菌进行基因分型、寄主转移和寄主专化性研究。9对微卫星引物将102株白僵菌分成31个微卫星基因型。在这31个微卫星基因型中,有5种基因型株系为相对优势菌株,这5种基因型株系通过侵染不同寄主昆虫即寄主转移延续自身在马尾松林生态系统中的传播和流行,从而证实寄主转移是白僵菌群体中的普遍现象。同时,这些相对优势基因型并不是在各个月份均匀分布,在大部分月份中,存在1–2种优势度高的基因型。相同基因型株系可侵染不同寄主的结果揭示出白僵菌不仅在种的水平,而且在菌株水平上寄主专化性也较弱。正是白僵菌较弱的寄主专化性特征促使部分基因型株系通过寄主转移,在马尾松林生态系统中得以宿存和延续。
Beauveria bassiana is one of the most common fungi, which has been widely used in pest biological control. 102 strains of Beauveria bassiana from Masson pine forest in Anhui Province were genotyped, host-transferred and host-specific by using simple sequence repeat (SSR) markers. Ninety pairs of microsatellite primers divided 102 strains of Beauveria bassiana into 31 microsatellite genotypes. Among the 31 microsatellite genotypes, 5 genotypes were relative dominant strains. These 5 genotypes continued their spread and spread in masson pine forest ecosystems by infecting different host insects, ie, host metastases , Confirming that host metastasis is a common phenomenon in Beauveria bassiana populations. At the same time, these comparative advantage genotypes are not evenly distributed in each month, and in most months there are 1-2 highly dominant genotypes. The results of different hosts infecting the same genotype revealed that Beauveria bassiana is not only species-specific but also host-specific at the strain level. It is the weaker donor-specific character of Beauveria bassiana that causes some of the genotypes to persist and continue in the Pinus massoniana forest ecosystem through host transfer.