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目的为探讨O6-甲基鸟嘌呤-DNA甲基转移酶(MGMT)基因多态性与新疆哈萨克族食管癌易感性的关系。方法运用病例-对照研究的方法,PCR-RFLP技术检测新疆哈萨克族食管癌51例及非癌对照109例的MGMT84C>T和MGMT135G>T单核苷酸多态(SNPs)的基因型。结果MGMT84C>T位点的三种基因型CC,CT,TT,在哈萨克族食管癌中所占比例分别为76.5%(39/51)、17.6%(9/51)、5.9%(3/51),对照组分别为77.0%(84/109)、18.3%(20/109)、4.6%(5/109),两组间分布差异不显著(P=0.938,P>0.05);MGMT135G>T位点的三种基因型GG,GT,TT,在哈萨克族食管癌中所占比例分别为90.2%(46/51)、7.8%(4/51)、2.0%(1/51),对照组分别为82.6%(90/109)、16.5%(18/109)、0.9%(1/109),两组间分布差异不显著(P=0.295,P>0.05);MGMT84C>T位点和MGMT135G>T位点联合分析,在食管癌组0突变等位基因与1-4突变等位基因所占比例分别为68.6%(35/51)、31.4%(16/51);对照组分别为:63.3%(69/109)、36.7%(40/109),两组间分布差异也不显著(P=0.511,P>0.05)。结论MGMT84C>T和MG-MT135G>TSNPs与哈萨克族食管癌易感性可能无关联。
Objective To investigate the relationship between the gene polymorphism of O6-methylguanine-DNA methyltransferase (MGMT) and susceptibility to esophageal cancer in Xinjiang Kazak. Methods The genotypes of MGMT84C> T and MGMT135G> T SNPs were detected by PCR-RFLP in 51 cases of Kazakh esophageal cancer and 109 cases of non-cancer control using the case-control study. Results The genotypes CC, CT, TT of MGMT84C> T loci were 76.5% (39/51), 17.6% (9/51) and 5.9% (3/51) respectively in Kazakh esophageal cancer ) In the control group were 77.0% (84/109), 18.3% (20/109) and 4.6% (5/109) respectively. There was no significant difference between the two groups (P = 0.938, P> 0.05) The three genotypes of GG, GT and TT in the Kazakh population were 90.2% (46/51), 7.8% (4/51) and 2.0% (1/51) respectively in the control group (P = 0.295, P> 0.05). The MGMT84C> T locus and MGMT135G were significantly higher than those in MGMT135G group (82.6%, 90/109, 16.5%, 0.9% > T locus, the percentage of 0 allele and 1-4 allele in esophageal cancer group were 68.6% (35/51) and 31.4% (16/51), respectively. The control group were: 63.3% (69/109) and 36.7% (40/109), respectively. There was no significant difference between the two groups (P = 0.511, P> 0.05). Conclusion MGMT84C> T and MG-MT135G> TSNPs may not be associated with susceptibility to Kazakh esophageal cancer.