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目的研究海南黎族、汉族高血压ACE基因多态性的相关性。方法采用聚合酶链反应(PCR)检测,对海南黎族111例高血压患者、146例黎族正常人、海南汉族106例高血压患者、97例汉族正常人的ACE基因插入/缺失(I/D)多态性检测,观察DD、DI、II基因型频率及等位基因频率,并对所有普通PCR定为DD型的样本进行插入特异性PCR检测,以减少误分型率。并调查经典危险因素。结果海南黎族高血压组DD、DI、II基因频率分别为10.8%、37.8%、51.4%;D及I等位基因频率分别为29.7%、70.3%。正常对照组DD、DI、II基因型频率分别为13.0%、43.8%、43.2%;D及I等位基因频率分别为34.9%、65.1%。两组之间DD、DI、II基因型频率及D、I等位基因频率均无显著性差异。汉族高血压DD、DI、II基因频率为16.0%、28.3%、55.7%;D及I等位基因频率分别为30.2%、69.8%。汉族正常人DD、DI、II基因频率15.5%、44.3%、40.2%,D及I等位基因频率分别为37.6%、62.4%。两组之间DD、DI、II基因型频率及D、I等位基因频率有显著性差异(P<0.05)。黎族、汉族高血压组与正常对照组比较,体重指数(BMI)、总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)有显著性差异(P<0.05);黎族高血压组收缩压,舒张压与正常对照组有显著性差异(P<0.05);汉族高血压组收缩压、舒张压与正常对照组有显著性差异(P<0.05)。结论在海南黎族高血压和黎族正常人的D等位基因频率均比I等位基因频率低;ACE基因I/D多态性与黎族高血压无显著关联;海南汉族高血压和汉族正常人D等位基因频率均比I等位基因频率低;ACE基因I/D多态性与汉族高血压的发病有相关性,是汉族高血压的主要致病基因,早期应用血管紧张素转化酶抑制剂的治疗干预。
Objective To investigate the association of ACE gene polymorphism with hypertension in Hainan and Han nationality. Methods Polymerase chain reaction (PCR) was used to detect the ACE gene insertion / deletion (I / D) in 111 cases of hypertension in Hainan Li, 146 cases of normal in Li, 106 cases of hypertension in Han nationality in Hainan, Polymorphism detection, DD, DI, II genotype frequencies and allele frequencies were observed. All PCR-specific PCR products were inserted into PCR-specific PCR to reduce the misdiagnosis rate. And investigate the classic risk factors. Results The frequency of DD, DI, and II genes in Hainan Li population were 10.8%, 37.8% and 51.4%, respectively. The frequencies of D and I alleles were 29.7% and 70.3%, respectively. The frequencies of DD, DI, and II genotypes in normal control group were 13.0%, 43.8% and 43.2%, respectively. The frequencies of D and I alleles were 34.9% and 65.1% respectively. There was no significant difference in the frequencies of DD, DI, II genotypes and D, I allele frequencies between the two groups. The frequencies of DD, DI and II genes of hypertension in Han were 16.0%, 28.3% and 55.7%, respectively. The frequencies of D and I alleles were 30.2% and 69.8% respectively. The frequencies of DD, DI and II genes in Han normal people were 15.5%, 44.3% and 40.2%, respectively. The frequencies of D and I alleles were 37.6% and 62.4% respectively. The frequencies of DD, DI and II genotypes and the frequencies of D and I alleles were significantly different between the two groups (P <0.05). The body mass index (BMI), total cholesterol (TC), triglyceride (TG) and low density lipoprotein cholesterol (LDL-C) in Li and Han hypertension group were significantly different from those in normal control group The systolic pressure and diastolic pressure of LiHypertension group were significantly different from those of normal control group (P <0.05). Systolic blood pressure and diastolic blood pressure of Han nationality hypertension group were significantly different from those of normal control group (P <0.05). Conclusion The frequency of D allele in Li nationality and Li nationality in Hainan province is lower than that in I allele. The ACE gene I / D polymorphism is not significantly associated with Li nationality hypertension. The Han nationality in Han nationality and Han normal population D Allele frequency is lower than I allele frequency; ACE gene I / D polymorphism is associated with the incidence of hypertension in Han Chinese hypertension is the main pathogenic genes early application of angiotensin converting enzyme inhibitors Therapeutic interventions.