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用密度梯度离心和ＤＮａｓｅ Ⅰ、ＲＮａｓｅ消化法从异源四倍体鲫鲤（Ｆ８）和鲫鲤Ｆ２肝组织中提取和纯化线粒体ＤＮＡ（ｍｔＤＮＡ）。用９种限制性内切酶对异源四倍体鲫鲤和鲫鲤Ｆ２的ｍｔＤＮＡ进行了分析，结果表明ＸｂａⅠ和ＢｇｌⅡ两种限制性内切酶在异源四倍体鲫鲤ｍｔＤＮＡ上存在酶切位点多态性。通过凝胶电泳测得异源四倍体鲫鲤ｍｔＤＮＡ相对分子量平均约 １０．２９ × １０６道尔顿，分子大小为 １６．２０ｋｂ；鲫鲤 Ｆ２ ｍｔＤＮＡ相对分子量为 １０． １８ × １０６道尔顿，分子大小为１６．０２ｋｂ。根据单酶解和双酶解的片段数目和分子大小，构建了异源四倍体鲫鲤ｍｔＤＮＡ的７种限制性内切酶（Ｋｐｎ Ⅰ、Ｐｓｔ、ＳａｌⅠ、ＸｈｏⅠ、Ｂｇｌ Ⅱ、ＢａｍＨⅠ和 ＸｂａⅠ）酶切图谱。
Mitochondrial DNA (mtDNA) was extracted and purified from allotetraploid crucian carp (F8) and crucian carp F2 liver tissue by density gradient centrifugation and DNase Ⅰ and RNase digestion. Nine kinds of restriction endonucleases were used to analyze the mtDNA of allotetraploid crucian carp and crucian carp F2, and the results showed that the two restriction enzymes Xba Ⅰ and Bgl Ⅱ allotetraploid mtDNA Cut site polymorphism. The average mtDNA molecular weight of allotetraploid crucian carp was about 10.29 × 106 Dalton by gel electrophoresis and the molecular size was 16.20kb. The relative molecular weight of F2 mtDNA in crucian carp was 10. 18 × 106 Daltons, the molecular size is 16.02kb. Seven restriction enzymes (Kpn Ⅰ, Pst, SalⅠ, XhoⅠ, Bgl Ⅱ, BamHⅠ and XbaⅠ) were constructed based on the number of single digested and double digested fragments and their molecular size. Digestion map.