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目的:研究脉络宁注射液对脂多糖(lipopolysaccharide,LPS)与角叉菜胶(carrageenan,Ca)联合诱导“热毒血瘀证”模型大鼠的影响。方法:随机将50只SPF级SD雄性大鼠分为5组,即空白对照组,模型对照组和脉络宁低、中、高剂量组(3.75,15,30 mg.kg-1)。空白对照组和模型对照组腹腔注射等容量的注射用生理盐水,脉络宁低、中、高剂量组分别腹腔注射脉络宁注射液(3.75,15,30 mg.kg-1),连续14 d,每天1次。给药到12 d,除空白组外,其余各组大鼠腹腔注射Ca(50 mg.kg-1)16 h后,再尾静脉注射LPS(5μg.kg-1),24 h后大鼠尾部出现淤血现象为模型建立成功。给药第14天,禁食12 h,自由饮水,10%水合氯醛麻醉大鼠,从腹主动脉取血和取肝、脾。竞争性抑制法检测血清白介素-6(IL-6),固相夹心法检测肿瘤坏死因子-α(TNF-α),检测全血黏度、血浆黏度、血浆凝血酶原时间(PT)和血小板聚集率,光镜下观察肝脏和脾脏的病理变化。结果:与模型组比较,脉络宁各剂量组的IL-6和TNF-α含量都相应减少(P<0.05),全血黏度、血浆黏度和血小板聚集率都降低(P<0.05或P<0.01),血浆凝血酶原时间增长(P<0.01),且呈量效依赖变化,肝脏和脾脏的炎症和淤血症状有一定的改善。结论:脉络宁注射液对LPS/Ca诱导“热毒血瘀证”模型大鼠的有一定的改善作用。
Objective: To study the effect of Mailuoning injection on the rats induced by lipopolysaccharide (LPS) combined with carrageenan (Carageenan) in “heat-toxic and blood-stasis syndrome” rats. Methods: Fifty SPF SD male rats were randomly divided into five groups: blank control group, model control group and Mailuoning low, medium and high dose groups (3.75,15,30 mg.kg-1). The normal control group and the model control group were given intraperitoneal injection of normal saline. Mailuoning low, medium and high dose groups were given intraperitoneal injection of Mailuoning injection (3.75,15,30 mg.kg-1) for 14 days, 1 day. The rats in the other groups were intraperitoneally injected with Ca (50 mg · kg-1) for 16 h after the administration of LPS (5 μg.kg-1) The phenomenon of congestion appears to be a successful model. On the fourteenth day after administration, fasted for 12 hours, freely drinking water, 10% chloral hydrate anesthetized rats, blood from the abdominal aorta and liver and spleen. Serum interleukin-6 (IL-6) was detected by competitive inhibition method. Tumor necrosis factor-α (TNF-α) was detected by solid phase sandwich method. Whole blood viscosity, plasma viscosity, plasma prothrombin time (PT) and platelet aggregation Rate, under the light microscope to observe the pathological changes of the liver and spleen. Results: Compared with the model group, the contents of IL-6 and TNF-α in Mailuoning group were decreased (P <0.05), and the whole blood viscosity, plasma viscosity and platelet aggregation rate were decreased (P <0.05 or P <0.01 ), Plasma prothrombin time increased (P <0.01), and the dose-dependent changes in the liver and spleen inflammation and congestion symptoms have some improvement. Conclusion: Mailuoning injection can improve LPS / Ca-induced “heat-induced blood stasis syndrome” model rats.