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ＨＥ染色法是组织学与病理学常用的染色方法，ＨＥ染色切片标本是学生观察最多的一种切片。但该切片经数年后，细胞易褪色，直接影响观察效果。本文结合多年实践经验与详细观察，就这一现象的成因与解决方法提出如下浅见。１褪色原因（１）封固剂的酸化问题：干性封固剂是最常
Hematoxylin and eosin staining is a common staining method in histology and pathology. Hematoxylin-eosin stained sections are the most observed sections of the students. However, after a few years, the cells are faded and have a direct impact on the observation effect. Combined with many years of practical experience and detailed observation, this article puts forward the following opinions on the causes and solutions of this phenomenon. 1 causes of fading (1) acidification of the sealing agent: dry mounting agent is the most common