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目的研究振动训练延缓废用性肌肉萎缩(SMDA)进程的分子机制。方法 SD雄性大鼠32只,随机均分为4组:正常对照组(A)、模型对照组(B)、每日振动训练组(C)和隔日振动训练组(D)。RT-PCR测比目鱼肌中IGF-1及Akt-1 mRNA的表达;Western-blotting测Akt-1和磷酸化Akt(pAkt-Thr308)的蛋白含量。结果 (1)与A组相比,B组、C组及D组右肢比目鱼肌湿重、肌肉湿重/体质量均显著下降(P<0.05)。(2)与A组比较,B组比目鱼肌IGF-1 mRNA、Akt-1 mRNA的表达和Akt-1蛋白含量均有显著性下降(P<0.05);与B组比较,C组和D组比目鱼肌IGF-1 mRNA、Akt-1 mRNA的表达和Akt-1蛋白含量均有显著性升高(P<0.05)。(3)与A组比较,B组和C组的Akt-1磷酸化水平均有下降,D组大鼠萎缩肌肉内磷酸化蛋白的表达水平显著下降(P<0.05)。结论振动训练可能通过IGF-1/PI3k/Akt1信号传导通路延缓SMDA,且不同间隔振动训练会对SMDA产生不同影响,其中每天振动训练干预的效果要优于隔天振动训练干预。
Objective To investigate the molecular mechanisms by which vibration training delays the process of disuse muscle atrophy (SMDA). Methods 32 SD male rats were randomly divided into 4 groups: normal control group (A), model control group (B), daily vibration training group (C) and alternate vibration training group (D). The expression of IGF-1 and Akt-1 mRNA in the soleus muscle was detected by RT-PCR. The protein levels of Akt-1 and phosphorylated Akt (pAkt-Thr308) were detected by Western-blotting. Results (1) Compared with group A, the soleus muscle wet weight and muscle wet weight / body weight of right leg in groups B, C and D were significantly decreased (P <0.05). (2) Compared with group A, the expression of IGF-1mRNA, Akt-1mRNA and Akt-1 protein in soleus muscle of group B were significantly decreased (P <0.05); Compared with group B, group C and group D The soleus muscle IGF-1 mRNA, Akt-1 mRNA expression and Akt-1 protein content were significantly increased (P <0.05). (3) Compared with group A, Akt-1 phosphorylation decreased in group B and group C, and the phosphorylated protein expression in atrophy of group D decreased significantly (P <0.05). Conclusion Vibration training may delay SMDA through IGF-1 / PI3k / Akt1 signal transduction pathway, and different intervals of vibration training will have different effects on SMDA. The daily vibration training intervention is better than the other day vibration training intervention.