论文部分内容阅读
目的研究二甲基亚砜(DMSO)对酵母多糖诱导的大鼠肾脏损伤的保护作用。方法将清洁级SD大鼠分为4组:SS组、SD组、ZS组及ZD组,每组各30只。ZS组和ZD组大鼠于腹腔注射750 mg/kg酵母多糖以制备肾损伤模型,SS组和SD组大鼠仅予以假手术操作。而后SD组与ZD组大鼠于1 h后皮下注射3 ml/kg DMSO,SS组与ZS组予以等剂量生理盐水。于造模后4、8、24 h分批处死大鼠,每批10只。采用激光多普勒血流仪测定各时间点肾血流量,并抽取腹主动脉血检测肌酐水平,取肾组织测定其含水率、丙二醛(MDA)及髓过氧化物酶(MPO)活性。结果造模后4、8、24 h,与SS组和SD组比较,ZS和ZD组肾血流量显著降低,肾组织含水率、MDA含量、MPO活性及血肌酐含量均显著升高(P均<0.05)。且与ZS组比较,ZD组造模后4、8、24 h肾血流量明显升高[(119±13)BPU vs.(156±22)BPU;(103±11)BPU vs.(121±12)BPU;(87±11)BPU vs.(108±11)BPU],肾MDA含量显著降低[(6.2±0.6)nmol/mg Pro vs.(5.3±0.4)nmol/mg Pro;(9.1±0.9)nmol/mg Pro vs.(5.6±0.5)nmol/mg Pro;(10.7±1.0)nmol/mg Pro vs.(6.7±0.6)nmol/mg Pro]。而ZD组肾含水率仅在8、24 h时[(75.7±2.2)%vs.(72.4±2.7)%;(79.2±2.3)%vs.(74.9±2.2)%],血肌酐含量仅在24 h时[(61.4±8.6)U/L vs.(36.5±6.7)U/L]较ZS组显著降低(P均<0.05)。但两组大鼠肾MPO活性在各时间点比较,差异均无统计学意义(P均>0.05)。结论DMSO对酵母多糖引起的肾缺血和氧自由基损伤有显著的保护作用。
Objective To investigate the protective effect of dimethyl sulfoxide (DMSO) on zymosan induced renal injury in rats. Methods Clean SD rats were divided into 4 groups: SS group, SD group, ZS group and ZD group, 30 rats in each group. Rats in ZS and ZD groups were injected intraperitoneally with 750 mg / kg zymosan to establish a renal injury model. Rats in SS group and SD group were only given sham operation. SD rats and ZD rats were injected with 3 ml / kg DMSO subcutaneously 1 h later, while SS rats and ZS rats were given equal doses of normal saline. Rats were sacrificed in batches 4, 8 and 24 h after modeling. Renal blood flow was measured at each time point by laser Doppler flowmetry, serum creatinine was measured by abdominal aorta blood test, and the content of water, malondialdehyde (MDA) and myeloperoxidase (MPO) . Results Compared with SS group and SD group, the renal blood flow rate, the content of MDA, the activity of MPO and the content of serum creatinine were significantly increased at 4, 8 and 24 h after modeling <0.05). Compared with the ZS group, the renal blood flow increased significantly at 4, 8 and 24 hours after modeling in the ZD group [(119 ± 13) BPU vs. (156 ± 22) BPU; (103 ± 11) BPU vs. (121 ± (6.2 ± 0.6) nmol / mg Pro vs. (5.3 ± 0.4) nmol / mg Pro; (9.1 ± 0.12) BPU; (87 ± 11) BPU vs. 0.9) nmol / mg Pro vs. (5.6 ± 0.5) nmol / mg Pro; (10.7 ± 1.0) nmol / mg Pro vs. (6.7 ± 0.6) nmol / mg Pro]. In the ZD group, the renal water content was only at 8 and 24 h [(75.7 ± 2.2)% vs (72.4 ± 2.7)%; (79.2 ± 2.3) vs (74.9 ± 2.2)%] (61.4 ± 8.6) U / L vs. (36.5 ± 6.7) U / L at 24 h were significantly lower than those in ZS group (all P <0.05). However, there was no significant difference in renal MPO activity between the two groups at all time points (all P> 0.05). Conclusion DMSO has a significant protective effect on renal ischemia induced by zymosan and oxygen free radical damage.