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[目的]筛选出慈姑茎尖组织培养的最佳试验条件。[方法]以江苏省慈姑的主栽品种“宝应紫圆”的茎尖为材料,研究了不同培养条件对慈姑茎尖培养和匍匐茎诱导的影响。[结果]0.1mg/L6-BA+0.05mg/LNAA培养条件下的成苗效果较好,2.00mg/L6-BA+0.50mg/LNAA培养条件对慈姑匍匐茎的诱导效果较好;不同蔗糖浓度处理间对慈姑抽生匍匐茎的影响差异不显著。[结论]不同培养条件对慈姑的成苗和匍匐茎诱导有一定影响。
[Objective] The research aimed to screen out the best experimental conditions of Jatropha shoot tissue culture. [Method] With the shoot tips of “Baoying Ziyuan”, a major cultivated variety of Sagittaria in Jiangsu Province as material, the effects of different culture conditions on shoot tip culture and stolonization induction were studied. [Result] The result of seedling establishment was better under the condition of 0.1mg / L 6-BA + 0.05mg / L NAA and the effect of 2.00mg / L 6-BA + 0.50mg / There was no significant difference in the effects of stoloniferous on arrowheads. [Conclusion] The different culture conditions had some effects on the induction of seedlings and stolons in Sagittaria.