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用 1 0g/ 10 0mL纤维素酶 (OnozukaR 10 ) ,0 1g/ 10 0mL果胶酶 (MecerozymeR 10 )及 10mmol/LCaCl2 ·2H2 O ,0 7mmol/LKH2 PO4 和 0 5mol/L甘露醇的混合酶液 ,从 14～ 18d苗龄的不结球白菜子叶上分离出高产率的原生质体。原生质体培养在KM8P1附加 2 ,4 D 0 5mg/L、 6 BA 0 2 5mg/L、NAA 0 5mg/L、葡萄糖 9 0g/ 10 0mL、蔗糖 1 0g/ 10 0mL、半乳糖0 0 3g/ 10 0mL液体培养基上 ,分裂旺盛。形成愈伤组织后经芽诱导和生根培养 ,获得了再生植株。
A mixture of 10 g / 10 mL of cellulase (Onozuka R 10), 0 1 g / 10 0 mL of pectinase (Mecerozyme R 10) and 10 mmol / L CaCl 2 · 2H 2 O, 0 7 mmol / L KH 2 PO 4 and 0 5 mol / L mannitol The protoplasts with high yield were isolated from 14-18 days old non-heading Chinese cabbage cotyledons. Protoplasts were cultured in the presence of KM8P1 supplemented with 2,4 D 0 5 mg / L, 6 BA 0 25 mg / L, NAA 0 5 mg / L, glucose 90 g / 100 mL, sucrose 1 0 g / 100 mL, galactose 0 0 3 g / 10 0mL liquid medium, strong division. Callus induction and rooting after callus formation resulted in regenerated plants.