目的从中国南海芋螺中寻找新的M家族芋螺毒素。方法应用3′RACE及巢式PCR进行基因克隆,将得到的目的基因连接入pGM-T载体、转化并测序,用进化分析软件对得到的新序列进行进化分析。结果从6种芋螺毒腺管中获得8个新的M家族芋螺毒素前体肽序列,其成熟肽含有15~21个氨基酸残基。结论这些新的M族毒素与已报道的M家族毒素序列同源性较低,是Mini-M家族的新成员。 Objective To search for a new M family conotoxin from Taro in South China. Methods The gene was cloned by 3’RACE and nested PCR. The target gene was ligated into pGM-T vector, transformed and sequenced. The evolutionary analysis software was used to analyze the new sequence. Results Eight new conotoxin precursor sequences of M family were obtained from six kinds of conotoxin pipes, and the mature peptide contained 15-21 amino acid residues. Conclusion These new class M toxins have low homology with reported M family toxins and are new members of the Mini-M family.