论文部分内容阅读
利用高效液相色谱法同时测定男壮胶囊中补骨脂素和异补骨脂素的含量。色谱条件：以μ－Ｂｏｎｄａ－ｐａｋ Ｃ１８为色谱柱，以甲醇－水（３５：６５）为流动相，检测波长２４５ｎｍ，柱温为３５±１℃。测得补骨脂素和异补骨脂素的线性范围分别为 ６～３０ ｍｇ／Ｌ（ｒ＝０．９９９９），５～２５ｍｇ／Ｌ（ｒ＝０．９９９９），平均回收率分别为９９．０％（ＲＳＤ＝１．８８％），１００．２％（ＲＳＤ＝１．１６％）。本法操作简便，结果准确，可作为男壮胶囊的质控方法。
Simultaneous determination of psoralen and isopsoralen in male Zhuang capsules by high performance liquid chromatography. Chromatographic conditions: μ-Bonda-pak C18 column with methanol-water (35:65) as the mobile phase, detection wavelength of 245 nm, and column temperature of 35±1°C. The linear ranges of psoralen and isopsoralen were 6-30 mg/L (r=0.9999) and 5-25 mg/L (r=0.9999), respectively. The average recoveries were 99. .0% (RSD = 1.88%), 100.2% (RSD = 1.16%). This method is simple, accurate, and can be used as a quality control method for male Zhuang capsules.