【摘 要】
：
利用组织培养和自毒物质根皮苷胁迫诱变相结合的方法,筛选了矮化砧木M26和八棱海棠抗自毒物质根皮苷突变体,并测定了突变体的生长发育情况和抗性生理指标。结果表明,根皮苷胁
【机 构】
：
河北农业大学国际合作处,河北农业大学植物保护学院,河北省农作物病虫害生物防治工程技术中心,
论文部分内容阅读
利用组织培养和自毒物质根皮苷胁迫诱变相结合的方法,筛选了矮化砧木M26和八棱海棠抗自毒物质根皮苷突变体,并测定了突变体的生长发育情况和抗性生理指标。结果表明,根皮苷胁迫筛选M26和八棱海棠抗性突变体的最佳选择压力分别为2 g/L和3 g/L,筛选到两株突变体B-1和M-1。根皮苷胁迫条件下,突变体B-1和M-1的分化稳定性较强,其株高、根长、鲜重等生长指标均明显高于野生型植株,且突变体根系的抗氧化酶SOD和CAT活性均明显高于野生型植株。
Using the combination of tissue culture and cortin glycoside stress mutagenesis, the mutants of dwarf rootstock M26 and anti-autotoxine substance Bombyx mori were screened for their growth and development and resistance Physiological indicators. The results showed that the optimal selection pressure for screening resistant mutants M26 and B. catenella were 2 g / L and 3 g / L, respectively, and two mutants B-1 and M-1 were screened. Under phlorizin stress, the mutants B-1 and M-1 had stronger differentiation stability and the growth indexes such as plant height, root length and fresh weight were significantly higher than those of wild-type plants, The activities of SOD and CAT were significantly higher than wild type plants.
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