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目的:观察酪氨酸激酶抑制剂STI571对骨髓瘤细胞系RPMI8226细胞增殖、细胞周期以及Rac1 mRNA表达的影响。方法:采用MTT法检测STI571对骨髓瘤细胞增殖的影响;流式细胞仪检测对细胞周期的影响;半定量RT-PCR研究Rac1mRNA水平的变化。结果:STI571明显抑制RPMI8226细胞的增殖,24、48、72 h IC50值分别为(34.52±2.31)μmol/L、(28.46±1.58)μmol/L、(25.74±2.44)μmol/L。25μmol/L STI571处理24 h,G1期细胞由55.8%增加至72.4%,S期细胞由34.8%减至15.6%。半定量RT-PCR显示,Rac1 mRNA水平在25μmol/L STI571处理24 h后明显下降。结论:STI571能抑制RPMI8226细胞的增殖,并可下调其Rac1 mRNA水平。
Objective: To observe the effect of tyrosine kinase inhibitor STI571 on proliferation, cell cycle and Rac1 mRNA expression in myeloma RPMI8226 cells. Methods: The effect of STI571 on the proliferation of myeloma cells was detected by MTT assay. The effect of STI571 on cell cycle was analyzed by flow cytometry. The level of Rac1 mRNA was detected by semi-quantitative RT-PCR. Results: STI571 significantly inhibited the proliferation of RPMI8226 cells. IC50 values at 24, 48 and 72 h were (34.52 ± 2.31) μmol / L, (28.46 ± 1.58) μmol / L and (25.74 ± 2.44) μmol / L, respectively. After treatment with 25μmol / L STI571 for 24 h, the percentage of cells in G1 phase increased from 55.8% to 72.4% and the percentage of S phase cells decreased from 34.8% to 15.6%. Semiquantitative RT-PCR showed that the Rac1 mRNA level decreased significantly after treated with 25μmol / L STI571 for 24 h. Conclusion: STI571 can inhibit the proliferation of RPMI8226 cells and down-regulate the expression of Rac1 mRNA.