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目的观察肿瘤坏死因子相关凋亡诱导配体(tumor necrosis factor related to apoptosis inducing ligand,TRAIL)联合白藜芦醇(resveratrol,Res)对人肝癌细胞凋亡的影响。方法流式细胞术检测细胞凋亡;CCK8法检测细胞增殖;Western blot检测细胞内Bax、Bcl-2蛋白的表达;分光光度法检测细胞内caspase3、caspase8的相对活性。结果 TRAIL联合Res作用Huh7细胞,联合用药组与对照组及单独用药组相比:肝癌细胞Huh7的凋亡率明显增加;促凋亡蛋白Bax表达明显增加,而凋亡抑制蛋白Bcl-2表达明显减少;caspase3、caspase8的相对活性明显升高。结论 TRAIL联合Res对诱导人肝癌Huh7细胞凋亡具有明显的协同效果,其机制可能与Bax表达增加、Bcl-2表达减少及caspase活性升高有关。
Objective To observe the effects of tumor necrosis factor related to apoptosis inducing ligand (TRAIL) and resveratrol (Res) on the apoptosis of human hepatoma cells. Methods Cell apoptosis was detected by flow cytometry. The proliferation of cells was detected by CCK8 assay. The expression of Bax and Bcl-2 protein was detected by Western blot. The relative activities of caspase3 and caspase8 were detected by spectrophotometry. Results Compared with the control group and the drug alone group, the apoptosis rate of Huh7 cells and the combination of TRAIL and Res treatment group were significantly increased: the apoptosis rate of Huh7 cells was significantly increased, the expression of Bax protein and Bcl-2 protein were significantly increased Reduce; caspase3, caspase8 relative activity was significantly increased. Conclusions TRAIL combined with Res has obvious synergistic effect on inducing apoptosis of human hepatoma Huh7 cells. The mechanism may be related to the increase of Bax expression, the decrease of Bcl-2 expression and the increase of caspase activity.