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四甲基偶氮唑盐 (MTT)比色法由于具有快速简便、无同位素污染等优点而被广泛应用于细胞增殖和细胞毒性试验[1] 。但不同细胞其生物学性状不同 ,所用细胞数量也应不同。我们以PC12和CHO细胞株为模型 ,于 2 0 0 3年 8— 9月对MTT比色法的条件及影响因素进行了探讨 ,寻求最佳条件
Tetramethylpyrazole (MTT) colorimetric method is widely used in cell proliferation and cytotoxicity test due to its advantages such as quick and easy, isotope-free contamination [1]. However, different cells have different biological traits, the number of cells used should also be different. We PC12 and CHO cell lines as a model, in August 2003 from August to September MTT colorimetric conditions and influencing factors were explored to find the best conditions