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目的探讨血清白细胞介素-8(IL-8)、白细胞介素-12(IL-12)及肿瘤坏死因子-α(TNF-α)在过敏性紫癜(HSP)患儿中表达及其临床意义。方法对本研究采用ELISA方法检测了48例过敏性紫癜患儿(其中22例合并肾脏损害)以及30例正常健康儿童的血清IL-8、IL-12及TNF-α水平,分别比较急性期和缓解期以及有无合并肾损害的过敏性紫癜患儿的细胞因子水平;分析IL-8、IL-12与TNF-α是否存在相关关系。结果HSP患儿血清IL-8、IL-12与TNF-α水平高于健康对照组(P<0.01);无肾损害HSP与紫癜性肾炎(HSPN)组血清IL-8、IL-12水平均无统计学差异;HSPN组肿瘤坏死因子-α水平高于无肾损害HSP组;HSP血清TNF-α水平与IL-8正相关(r=0.524P<0.01)。HSP患儿血清TNF-α水平与IL-12正相关(r=0.670,P<0.01)。结论细胞因子IL-8、IL-12及TNF-α可能参与HSP/HSPN发病过程。
Objective To investigate the expression of interleukin-8, interleukin-12 (IL-12) and tumor necrosis factor-α (TNF-α) in children with Henoch-Schonlein purpura (HSP) and its clinical significance . Methods Serum levels of IL-8, IL-12 and TNF-α in 48 children with HSP (22 with renal impairment) and 30 healthy children were detected by ELISA in this study. The acute and remission stages were compared respectively. And whether there is renal damage in children with Henoch-Schonlein purpura cytokine levels; analysis of IL-8, IL-12 and TNF-α whether there is a correlation. Results Serum levels of IL-8, IL-12 and TNF-α in children with HSP were significantly higher than those in healthy controls (P <0.01). Serum levels of IL-8 and IL-12 in HSP- The levels of tumor necrosis factor-α in HSPN group were higher than those in HSP group without renal damage. The level of TNF-α in HSP serum was positively correlated with IL-8 (r = 0.524 P <0.01). Serum TNF-α levels in children with HSP were positively correlated with IL-12 (r = 0.670, P <0.01). Conclusion The cytokines IL-8, IL-12 and TNF-α may be involved in the pathogenesis of HSP / HSPN.