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目的探讨乌司他丁(Ulinastatin,U)对大鼠在体肺缺血-再灌注损伤中炎症反应的影响。方法 SD大鼠36只随机分成6组:①假手术组。②单纯缺血0.5h组。③缺血-再灌注0.5h组。④缺血-再灌注2h组。⑤缺血-再灌注0.5h+U治疗组。⑥缺血-再灌注2h+U治疗组。测定各组肺组织中白介素-10(IL-10)及血浆可溶性细胞间黏附分子-1(sICAM-1)的水平,测定肺组织的通透性(125I标记牛血清白蛋白的漏出量表示)。结果肺缺血-再灌注损伤组肺组织中sICAM-1水平明显高于假手术组和缺血组(P<0.01),IL-10水平亦明显高于假手术组(P<0.01),肺组织的通透性比假手术组和缺血组明显升高(P<0.01),组织损伤明显。应用U后,血浆sICAM-1水平明显低于相应缺血再灌注各时间点(P<0.01),而IL-10水平亦明显高于相应缺血再灌注各时间点(P<0.01),肺组织的通透性明显低于缺血-再灌注损伤组(P<0.01)。结论 U可能通过抑制炎症反应从而减轻肺缺血-再灌注损伤。
Objective To investigate the effect of Ulinastatin (U) on the inflammatory reaction in the rat lung after ischemia-reperfusion injury. Methods Thirty - six SD rats were randomly divided into 6 groups: ① sham operation group. ② ischemia 0.5h group. ③ ischemia - reperfusion 0.5h group. ④ ischemia - reperfusion 2h group. ⑤ ischemia - reperfusion 0.5h + U treatment group. ⑥ ischemia - reperfusion 2h + U treatment group. The levels of interleukin-10 (IL-10) and soluble intercellular adhesion molecule-1 (sICAM-1) in the lung tissue of each group were measured and the permeability of lung tissue was measured (leakage of 125I labeled bovine serum albumin) . Results The level of sICAM-1 in lung tissue of lung ischemia-reperfusion injury group was significantly higher than that in sham-operation group and ischemia group (P <0.01), IL-10 level was also significantly higher than that in sham operation group (P <0.01) Tissue permeability than the sham operation group and ischemia group was significantly higher (P <0.01), tissue damage significantly. After application of U, plasma sICAM-1 levels were significantly lower than those at the corresponding time point of ischemia-reperfusion (P <0.01), and IL-10 levels were also significantly higher than those of the corresponding ischemia-reperfusion groups Tissue permeability was significantly lower than ischemia-reperfusion injury group (P <0.01). Conclusion U may reduce lung ischemia-reperfusion injury by inhibiting the inflammatory response.