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聚乙烯吡咯烷酮(PVP)是一种重要的非渗透性低温保护剂。本文使用低温显微镜,研究了不同浓度(10%、20%和30%)PVP水溶液在不同降温速率(1℃/min、10 ℃/min、50℃/min和100℃/min)的结晶形态以及初始结晶温度。研究结果表明,在较低浓度条件下结晶颗粒较大,容易出现冰沟壑;在较高浓度条件下,冰晶颗粒相对较小,未见明显的冰沟壑产生;此外,初始结晶温度随着降温速率的增加而降低。在适当的高浓度条件下提升降温速率可以有效减少结晶量,有利于低温保存。
Polyvinylpyrrolidone (PVP) is an important non-permeable cryo-protectant. In this paper, we investigated the crystalline morphology of PVP aqueous solutions with different cooling rates (1 ℃ / min, 10 ℃ / min, 50 ℃ / min and 100 ℃ / min) with different concentration (10%, 20% and 30% Initial crystallization temperature. The results show that the ice crystals are more likely to appear at lower concentration and the ice crystals are more likely to appear at higher concentration. There is no obvious glacial gully generation. In addition, the initial crystallization temperature decreases with the cooling rate Increase and decrease. In the appropriate conditions of high concentration to enhance the cooling rate can effectively reduce the amount of crystallization, is conducive to low temperature preservation.