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为了进一步了解弓形体在我省人畜中的感染情况,1985年我们继续采用间接血凝试验(IHA),对3市5县作血清流行病学调查,结果如下。 材料和方法 一、抗原制备 (一)虫株传代:弓形体长宁株,上海市农科院畜牧兽医研究所提供。取20～25g小白鼠经腹腔接种虫株,3天后剖杀,注入腹腔PBS2ml,抽取腹水转种传代,不用时置液氮深冷保存。 (二)抗原处理:按上海市农科院畜牧兽医研究所介绍的方法,抽取腹水、洗涤、胰酶消化、低温冻融而获得。 二、血球制备 (一)固定羊血球:采年青公羊血,常规处理后用2.5％戊二醛醛化1小时,洗涤后即为固定羊血球。
In order to further understand the Toxoplasma gondii infection in our province in humans and animals, we continue to use indirect hemagglutination test (IHA) in 1985, three cities and 5 counties for serological epidemiological survey results are as follows. Materials and methods First, the antigen preparation (A) insect strain passage: Toxins Changning strain, Shanghai Academy of Agricultural Sciences Animal Husbandry and Veterinary Institute. Take 20 ~ 25g mice were intraperitoneally inoculated with insect strains, 3 days after the cut, into the peritoneal PBS2ml, ascites ascites subcultured, when not placed in liquid nitrogen cryopreservation. (B) antigen processing: According to the Shanghai Academy of Agricultural Sciences Animal Husbandry and Veterinary Institute introduced the method of ascites, washing, trypsin digestion, low temperature freeze-thaw and access. Second, the preparation of blood cells (a) fixed sheep blood cells: mining young ram blood, conventional treatment with 2.5% glutaraldehyde hydroformylation 1 hour after washing is fixed sheep blood cells.