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目的探讨舒血宁注射液对高碘致人脐静脉血管内皮细胞损伤的保护作用。方法体外培养人脐静脉血管内皮细胞株HUECV-304,在培养液中预先加入不同浓度的碘化钾和舒血宁注射液。在碘化钾的最终浓度分别为4.0、5.0、6.0、8.0μmol/L组中分别加入浓度为0、2、5、10μL/mL的舒血宁注射液,观测4,8,12和24 h时细胞形态、细胞计数、MTT法测定细胞活性及上清液中SOD的含量。结果①在碘浓度5.0μmol/L以上组,4 h时可见内皮细胞内有中毒颗粒出现,且随浓度增加和时间延长而增加。加入舒血宁后,碘浓度5.0μmol/L以上组该变化明显减轻,且与时间和剂量相关(P<0.05);②随时间的延长及舒血宁浓度的升高,细胞增殖活性受到抑制的碘浓度相应升高(P<0.05);③SOD含量随碘浓度的升高而升高(P<0.05),加入舒血宁后,SOD含量变化不明显。结论舒血宁注射液对高碘所引起的内皮细胞损伤有一定的对抗作用,其作用存在时间-剂量效应关系;舒血宁的作用可通过增强细胞的抗氧化作用拮抗高碘的损伤作用。
Objective To investigate the protective effect of Shuxuening injection on human umbilical vein endothelial cell injury induced by high iodine. Methods Human umbilical vein endothelial cell line HUECV-304 was cultured in vitro. Different concentrations of potassium iodide and Shuxuening injection were added to the culture medium in advance. In the final concentrations of potassium iodide 4.0, 5.0, 6.0, and 8.0 μmol/L, Shuxuening injection was added at concentrations of 0, 2, 5, and 10 μL/mL, and cells were observed at 4, 8, 12 and 24 h. The morphology, cell count, and MTT assay were used to determine cell viability and SOD content in the supernatant. Results 1 In the iodine concentration above 5.0μmol/L group, there were toxic particles appearing in the endothelial cells at 4h, which increased with the increase of concentration and time. After the addition of Shuxuening, the change of iodine concentration in the group of 5.0μmol/L or more was significantly reduced and correlated with time and dose (P<0.05); 2 The proliferative activity was inhibited with the prolongation of time and the increase of the concentration of Shuxuening The iodine concentration increased correspondingly (P<0.05); 3SOD content increased with the increase of iodine concentration (P<0.05). After adding Shuxuening, the SOD content did not change significantly. Conclusion Shuxuening injection has a certain antagonistic effect on endothelial cell damage caused by high iodine, and its action has a time-dose effect relationship. The effect of Shuxuening can antagonize the injury effect of high iodine by enhancing the antioxidation of cells.