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目的:检测早发型子痫前期患者与正常晚期妊娠妇女血清及胎盘组织中胎盘生长因子(PLGF)及一氧化氮(NO)的表达情况,并探讨两者在早发型子痫前期中的作用及相互关系。方法:将60例妊娠孕妇随机分为3组,A组20例,为正常晚期妊娠孕妇;B组20例,为轻度早发型子痫前期孕妇;C组20例,为重度早发型子痫前期孕妇。用酶联免疫吸附法(ELISA)检测3组孕妇血清中PLGF、NO浓度的变化。用免疫组化法检测3组孕妇胎盘组织中PLGF、NO浓度的变化。结果:B组、C组血清及胎盘组织中PLGF、NO水平均明显低于A组(P<0.05),且随早发型子痫前期病情的加重,PLGF、NO的水平也随之降低,各组两两比较有显著性差异(P<0.05)。B组、C组患者血清与胎盘组织中PLGF水平分别与相应部位的NO水平正相关(r=0.424,0.389,P均<0.05)。结论:孕妇血清及胎盘组中PLGF、NO水平变化可能与早发型子痫前期孕妇发病及病情发展密切相关,且可作为监测患者病情变化的参考指标。
Objective: To detect the expression of placental growth factor (PLGF) and nitric oxide (NO) in serum and placenta of early-onset preeclampsia and normal late pregnant women, and to explore their roles in early-onset preeclampsia and Interrelationship. Methods: 60 pregnant women were randomly divided into 3 groups, 20 cases in group A were normal pregnant women of late pregnancy; 20 cases in group B were mild premature preeclampsia; 20 cases in group C were severe premature eclampsia Pre-pregnant women. Serum levels of PLGF and NO in three groups of pregnant women were detected by enzyme-linked immunosorbent assay (ELISA). Immunohistochemistry was used to detect the changes of PLGF and NO in the placenta of three groups. Results: The levels of PLGF and NO in serum and placenta of group B and group C were significantly lower than those of group A (P <0.05). The levels of PLGF and NO were also decreased with the onset of preeclampsia There was a significant difference between groups (P <0.05). PLGF levels in serum and placenta of group B and group C were positively correlated with NO levels of corresponding sites (r = 0.424,0.389, P <0.05). Conclusion: The changes of PLGF and NO in serum and placenta of pregnant women may be closely related to the onset and progression of preeclampsia in pregnant women and may be used as a reference index to monitor the changes of patients’ condition.