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棉花属种间远缘杂交,当代种子由于亲和力弱,往往只发育种壳,而不发育胚珠,成为空粒,或者胚珠中途停育,变成瘪粒,实际上结实率甚低。如何正确鉴定其结实率,判断其种间远缘杂交亲和力,过去没有一个比较理想的手段,国内外也没有这方面的报道。近三年来,我们应用软X射线摄影方法,来诊断棉花远缘杂种当代种子胚珠的发育程度,取得较好的结果。 (一)材料方法供试材料:中棉(G.arboreum)大篰落树×戴维逊(G.davi Sonii);中棉(G.arboreum)常熟黑籽×陆地棉(G.hirsutum)岱字15号等8个组合,9份材料;共250粒杂交种子。仪器:DGX-4
Cotton is a distant hybrid between species. Because of their weak affinity, modern seeds tend to develop only the seed coat, but not the ovules. They become empty grains, or the ovules stop midway and turn into shriveled grains. In fact, the seed setting rate is very low. How to correctly identify its seed setting rate, to determine the inter-species hybridization affinity affinity, in the past there is not a more ideal means, there is no report at home and abroad. In the past three years, we have used soft X-ray photography to diagnose the development of ovules in contemporary cotton distant hybrids and achieved good results. (A) material methods to test materials: cotton (G.arboreum) × davidius David (G.davi Sonii); cotton (G.arboreum) Changshu black seed × G. cotton (G.hirsutum) Dai Word 15 and other 8 combinations, 9 materials; a total of 250 hybrid seeds. Instrument: DGX-4