目的　研究HER 2 /neu(c erbB 2 )在结、直肠恶性肿瘤中的表达及其与肿瘤分期的相关性。方法　收集 2 0 0 2年 1月至 2 0 0 3年 5月治疗的 2 8例结、直肠恶性肿瘤的临床资料。按Dukes分期标准进行分期 ,其中A、B期共 17例 (17/2 8,6 0 7% ) ,C、D期 11例 (11/2 8,39 3% )。采用HER 2单克隆抗体试剂套盒行免疫组织化学染色 ,并以染色细胞数目的多少及染色程度为分级标准 ,认为 2 +以上 (含 2 +)为过表达 (报告为 +～ ++的 ,记为阴性 )。结果　该蛋白在A、B期肿瘤中的过表达率为 11 8% (2 /17) ;C、D期肿瘤中过表达率为 5 4 5 % (6 /11)。该蛋白的过表达率在有转移和无转移的病例之间差异有显著意义 (P <0 0 5 )。结论　虽然在结、直肠恶性肿瘤病例中HER 2 /neu的过表达率不高 ,但仍提示HER 2 /neu过表达程度与肿瘤的进展或侵袭性生物学行为相关。 Objective To study the expression of HER 2 / neu (c erbB 2) in the malignant tumors of the knot and rectum and its correlation with tumor staging. Methods The clinical data of 28 patients with colorectal cancer who were treated from January 2002 to May 2003 were collected. According to Dukes staging criteria, there were 17 cases (17/2 8, 607%) in stage A and B, and 11 cases (11/2 8, 39 3%) in stage C and D. HER2 monoclonal antibody kit kit immunohistochemical staining and the number of stained cells and the degree of staining for the grading standards that 2 + or more (including 2 +) is overexpressed (reported as ~ ~ ++, Recorded as negative). Results The overexpression rate of this protein was 11 8% (2/17) in stage A and B tumors. The overexpression rate was 54.5% (6/11) in stage C and D tumors. The protein overexpression rate was significantly different between cases with metastasis and without metastasis (P <0.05). Conclusions Although the overexpression of HER 2 / neu is not high in cases of colorectal malignant neoplasms, it is suggested that the overexpression of HER 2 / neu may be related to tumor progression or aggressive biological behavior.