1 材料 本文的清肝剂是由本文的作者金泰日等对牛胆汁和黑豆进行生物转化后,加复合酶提取的有效成分。RPMI-1940,日本株式会社产品;胰蛋白酶,美国DIFCO公司产品;熊胆粉,由延吉市珍贵动物饲养场提供;WISTAR乳鼠由我院动物科提供。2 方法 组织化学方法:将采用姜玉顺等的方法培养的Wister乳鼠原代单层肝细胞40瓶分为正常组、损伤组、熊胆粉 1 Materials The hepatophagic agent of this article is the active ingredient extracted by the complex enzyme after the biotransformation of bovine bile and black bean by the author Jin Tairi et al. RPMI-1940, a product of Japan Co., Ltd.; trypsin, a product of the US DIFCO company; bear bile powder, provided by Yanji City, a precious animal farm; WISTAR suckling mice are provided by the Department of Zoology of our hospital. 2 Methods Histochemical methods: 40 bottles of primary monolayer hepatocytes of Wistar pups, which were cultured by methods such as Jiang Yushun, were divided into normal group, injury group and bear bile powder.