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目的 :调查兰州地区乙肝病毒基因分型情况及其和性别、血清标志物的关系。方法 :用PCR 微板核酸杂交 ELISA法对 88例兰州地区慢性无症状携带者 (AsC)及慢性乙型肝炎 (CHB)、肝炎后肝硬化 (HLC)的HBVDNA进行基因分型 ,并用酶免疫法 (EIA)测其血清标志物 (HBV M )。结果 :兰州地区基因型C型 67 0 % ,B型 12 5 % ,B、C混合型18 2 % ,其他型 2 3 %。男、女性感染HBVDNA构成比存在差异 ,HBeAg阳性率C型较B型高。结论 :兰州地区的基因型以C型为主 ,乙肝病毒基因亚型易感性可能存在性别差异 ,不同基因亚型感染可形成不同的血清标志物。
Objective: To investigate the genotypes of hepatitis B virus in Lanzhou and its relationship with gender and serum markers. Methods: Eighty-eight cases of Asymptomatic carriers of asymptomatic Asc and Chronic Hepatitis B (CHB) and Hepatitis cirrhosis (HLC) in 88 cases of Lanzhou were genotyped by using PCR microplate nucleic acid hybridization ELISA. Enzyme-linked immunosorbent assay (EIA) measured serum markers (HBV M). Results: In Lanzhou, genotype C was 67 0%, type B 125%, type B and C mixed 182%, and other types 23%. The ratio of HBVDNA infection between male and female is different, and the positive rate of HBeAg is higher than that of B type. Conclusion: The genotypes of Lanzhou are mainly C genotype. There may be gender differences in the susceptibility of hepatitis B virus subtypes. Different serotypes of different genotypes may form different serum markers.