[目的 ]探讨香烟对生殖作用的影响。 [方法 ]腹腔注射香烟烟雾水溶物后观察其对小鼠怀孕率、胚胎发育的影响 ,采用卵母细胞的体外培养和体外受精的方法 ,观察其对超排卵数、卵母细胞的成熟和受精能力影响。 [结果 ]香烟烟雾水溶物可以极显著地降低小鼠的妊娠率 ,抑制胚胎发育 ,还可以显著性地抑制卵母细胞的体内成熟 ,降低第一极体的释放率和卵母细胞的体外受精率 ,使卵母细胞在体外的生存能力明显降低。本实验结果还显示 ,香烟烟雾水溶物对小鼠生殖作用的损害和对卵母细胞成熟的抑制作用是可逆的 ,停止腹腔注射香烟烟雾水溶物和将卵母细胞或受精卵培养在正常的培养液中 ,小鼠的生育能力又能恢复、卵母细胞的第一极体的释放率以及体外受精率都明显提高。[结论 ]香烟烟雾水溶物可以破坏卵母细胞的成熟分裂 ,降低小鼠的生殖能力和生育能力 ,但这种毒性作用是可以逆转的 ,戒烟后又可恢复正常 [Objective] To investigate the effect of cigarette on reproductive function. [Method] The effects of intraperitoneal injection of cigarette smoke and water soluble substance on the pregnancy rate and embryo development of mice were observed. The oocyte in vitro culture and in vitro fertilization were used to observe the effects on the number of superovulation, oocyte maturation and fertilization Ability to influence. [Result] Cigarette smoke water soluble substance could remarkably reduce the pregnancy rate, inhibit embryonic development, inhibit the maturation of oocytes in vivo, reduce the release rate of the first polar body and in vitro fertilization of oocytes Rate, significantly reduce the viability of oocytes in vitro. The results of this experiment also showed that the harm of the cigarette smoke water soluble substance to the reproductive function of mice and the inhibitory effect on the maturation of the oocyte are reversible, stopping the intraperitoneal injection of the cigarette smoke water soluble substance and culturing the oocyte or fertilized egg in the normal culture Liquid, the reproductive capacity of mice and can recover, the first polar body of the oocyte release rate and in vitro fertilization rates were significantly improved. [Conclusion] Cigarette smoke and water soluble substances can destroy the oocyte maturation and division and reduce the mice reproductive capacity and fertility, but this toxic effect can be reversed, and then returned to normal after quitting smoking