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目的 :1995年山西晋南地区发生恙虫病流行 ,我们对该地区人群进行了流行病学调查 ;并对从患者和野鼠体内分离的恙虫病立克次体进行了病原学研究。方法 :利用微量间接免疫荧光法(MIF)对恙虫病立克次体在人群中的流行进行了调查 ;采用MIF、PCR/RFLP和序列分析方法对恙虫病立克次体分离株进行了鉴定。结果和结论 :晋南地区人群血清恙虫病立克次体抗体阳性率为2 5% ;恙虫病立克次体山西分离株 (Sxh951,Sxh952 ,Sxh953,Sxm97)的血清型别为Gilliam株 ;序列分析提示 :在相对分子质量 560 0 0蛋白基因水平上 ,Sxh951株与Yongchon株亲缘关系最近。
OBJECTIVE: Epidemiology of scrub typhus occurred in the southwestern Shanxi Province in 1995. Epidemiological surveys were conducted among the populations in this area. Etiological research was conducted on rickettsia tsutsugamushi isolated from patients and wild mice. Methods: The epidemic situation of Rickettsia tsutsugamushi Rickettsia was investigated by micro indirect immunofluorescence assay (MIF). Rickettsia tsutsugamushi isolates were identified by MIF, PCR / RFLP and sequence analysis. RESULTS AND CONCLUSION: The positive rate of Rickettsia tsutsugamushi antibody was 25% in populations of southern Shanxi Province. The serotype of Rickettsia tsutsugamushi isolates from Shanxi Province (Sxh951, Sxh952, Sxh953, Sxm97) was Gilliam strain. The sequence The analysis suggests that the Soxh951 strain has the closest genetic relationship with the Yongchon strain at the molecular level of 560 0 0.