利用逆转录病毒中介基因转移体系将ｈＧＭ—ＣＳＦ基因导入大鼠Ｗａｌｋｅｒ瘤细胞ＷＴ２５６中，经ＰＣＲ和ＳｏｕｔｈｅｒｎＢｌｏｔ检测证实，ｈＧＭ—ＣＳＦ基因已成功导入ＷＴ２５６细胞，生物学活性检测表明，转基因瘤细胞分泌一定水平ｈＧＭ—ＣＳＦ。转基因细胞经Ｘ线灭活，接种荷瘤的Ｗａｌｋｅｒ瘤模型，诱导了有效的抗肿瘤免疫反应 The hGM-CSF gene was introduced into rat Walker tumor cells WT256 using a retrovirus-mediated gene transfer system. PCR and SouthernBlot analysis confirmed that the hGM-CSF gene was successfully introduced into WT256 cells. Biological activity assay showed that the transgenic tumor cells secreted certain Horizontal hGM—CSF. Transgenic cells were inactivated by X-ray and inoculated tumor-bearing Walker tumor models to induce effective antitumor immune responses