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以优良玉米自交系综3为供体、87-1为受体,利用回交和SSR标记辅助选择相结合的方法,获得了51份纯合的染色体单片段代换系(Single Segment Substitution Line,SSSL),每个SSSL内只含有一个来源于供体的染色体片段。51个代换片段不均匀分布在玉米10条染色体上,代换片段长度在1.75～172.45 cM之间,平均长度为24.44cM。覆盖玉米基因组的总长度为933.90 cM,覆盖率为40.20%。2008年在郑州利用17份SSSL材料及在三亚利用39份SSSL材料对玉米穗长进行了1年2点的表型鉴定,共检测到20个穗长QTL,加性效率百分率在-12.10%～19.18%之间。结果表明,这些单片段代换系存在较大的遗传变异,是用于玉米复杂性状的QTL鉴定、基因克隆及功能分析的理想材料。
Using 51 maize inbred lines as the donor and 87-1 as the donor, the combination of backcross and SSR marker-assisted selection provided 51 pairs of single Segment Substitution Line , SSSL) contains only one donor-derived chromosomal fragment per SSSL. 51 substitutional fragments were unevenly distributed on 10 chromosomes of maize. The length of the substitution fragment was between 1.75 and 172.45 cM with an average length of 24.44 cM. The total length of the maize genome was 933.90 cM with a coverage of 40.20%. In 2008, 17 SSSL materials were used in Zhengzhou and 39 SSSL materials were used in Sanya for 1 year and 2-point phenotypic identification. A total of 20 ear length QTLs were detected, with the additive efficiency percentage ranging from -12.10% 19.18% between. The results showed that there was a large genetic variation among these single-segment substitution lines and was an ideal material for QTL identification, gene cloning and functional analysis of complex traits in maize.