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目的:通过观察肺纤方干预肺间质纤维化大鼠肺微血管内皮细胞体外培养的影响,探讨肺纤方抗肺间质纤维化微血管内皮细胞黏附及游走的机制。方法:从肺纤维化模型组大鼠分离培养肺微血管内皮细胞。将其等量分为空白对照组,氯沙坦(10μg/m L)组,泼尼松(5μg/m L)组,肺纤方大、中、小剂量(100、60、20μg/m L)组。WST-1法检测肺微血管内皮细胞的生长黏附率。Transwell法检测肺微血管内皮细胞的迁移细胞进行计数。结果:与空白对照组、泼尼松组比较,肺纤方大、中剂量和氯沙坦可以显著抑制肺微血管内皮细胞的黏附率,作用48h尤为明显(P<0.01)。与空白对照组、泼尼松组比较,肺纤方大、中剂量和氯沙坦可抑制内皮细胞的迁移(P<0.01)。结论:肺纤方可以显著抑制肺间质纤维化大鼠肺微血管内皮细胞的黏附和游走迁移,从而具有抗肺间质纤维化作用。
OBJECTIVE: To investigate the effect of Fei Xian Fang on pulmonary microvascular endothelial cells (PEI) cultured in vitro in rats with pulmonary fibrosis, and to explore the mechanism of Fei Xian Fang’s resistance to pulmonary interstitial fibrosis and endothelial cell adhesion and migration. Methods: Pulmonary microvascular endothelial cells were isolated and cultured from pulmonary fibrosis model rats. The rats were randomly divided into blank control group, losartan (10μg / m L) group, prednisone (5μg / m L) )group. WST-1 method was used to detect the growth and adhesion rate of pulmonary microvascular endothelial cells. Transwell method was used to detect the migration of pulmonary microvascular endothelial cells for counting. Results: Compared with the blank control group and the prednisone group, the pulmonary fibrosis large and medium dose and losartan significantly inhibited the adhesion rate of pulmonary microvascular endothelial cells, especially for 48h (P <0.01). Compared with the blank control group and the prednisone group, the pulmonary fibrosis large and medium dose and losartan can inhibit the migration of endothelial cells (P <0.01). Conclusion: Feixian Prescription can significantly inhibit the adhesion and migration of pulmonary microvascular endothelial cells in pulmonary interstitial fibrosis in rats and thus have anti-pulmonary interstitial fibrosis.