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为了评价益生菌制剂对腹泻模型小鼠肠道菌群的恢复,现采用22.4 g/(kg·d)氨苄青霉素灌胃构造小鼠腹泻模型,其中空白对照组灌胃等量生理盐水。造模成功后,用低、中、高不同剂量的益生菌制剂对小鼠治疗恢复,治疗14 d后,观察益生菌制剂对小鼠稀便率、稀便级和腹泻指数的影响。无菌取治疗后0、7、14 d的小鼠粪便进行分析,分别采用平板计数和DGGE技术检测肠道目标菌群变化。停用益生菌制剂后,无菌采取0、7、14 d的粪便样品,荧光定量PCR定量检测外源益生菌。结果表明,益生菌制剂不仅能改善小鼠的腹泻情况,促进腹泻小鼠的生长,还对腹泻小鼠肠道菌群结构具有显著恢复作用;荧光定量PCR检测结果表明外源益生菌嗜酸乳杆菌NCFM、乳双歧杆菌Bi-07和鼠李糖乳杆菌HN001在停止灌胃益生菌制剂后可定殖存活,但随着时间推移呈一定衰减的趋势。
To evaluate the recovery of intestinal microflora by probiotics in diarrhea model mice, intraperitoneal injection of ampicillin at a dose of 22.4 g / (kg · d) was used to establish mice diarrhea model. The blank control group was given the same amount of saline. After successful modeling, the mice were treated with different doses of probiotics in low, medium and high doses. After 14 days of treatment, the effect of probiotic preparations on the rate of loose stools, loose stools and diarrhea index in mice was observed. The stool samples of mice at 0, 7 and 14 days after aseptic treatment were analyzed. The changes of intestinal flora were detected by plate count and DGGE respectively. After stopping the preparation of probiotics, stool samples were aseptically taken for 0, 7 and 14 days, and exogenous probiotics were quantitatively detected by fluorescence quantitative PCR. The results showed that probiotics not only can improve the diarrhea in mice, promote the growth of diarrhea mice, but also have a significant recovery effect on the structure of intestinal flora in diarrhea mice. The results of real-time quantitative PCR showed that the exogenous probiotics yoghurt Bacillus NCFM, Bifidobacterium lactis Bi-07 and Lactobacillus rhamnosus HN001 colonized surviving probiotics, but tended to decay with time.