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目的观察体外注射重组人促红细胞生成素(rhEPO)对大鼠脑外伤脑组织线粒体ATP酶活性的影响,探讨rhEPO对脑外伤后神经保护作用的机制。方法建立大鼠自由落体脑挫裂伤模型,伤后立即腹腔注射rhEPO,采用改良Lowry氏法分别测定治疗后4、12、24和48 h及各自对照组大鼠脑组织线粒体Na+-K+ATP酶、Ca2+-ATP酶及Mg2+-ATP酶活性。结果脑外伤后大鼠脑组织线粒体Na+-K+ATP酶、Ca2+-ATP酶及Mg2+-ATP酶活性均显著下降(P<0.05)。rhEPO治疗后12、24和48 h脑组织线粒体ATP酶活性均显著高于各自时间点对照组(P<0.05)。结论外源性rhEPO可通过影响线粒体功能而减轻脑外伤后的继发性脑损害,从而改善预后。
Objective To observe the effects of rhEPO on mitochondrial ATPase activity in rat brain after traumatic brain injury and to explore the neuroprotective mechanism of rhEPO after traumatic brain injury. Methods The rat model of cerebral contusion and laceration was established by intraperitoneal injection of rhEPO immediately after injury. The levels of mitochondrial Na + -K + ATP in rat brain at 4, 12, 24 and 48 h after treatment were measured by modified Lowry’s method Enzyme, Ca2 + -ATPase and Mg2 + -ATPase activity. Results The activities of mitochondrial Na + -K + ATPase, Ca2 + -ATPase and Mg2 + -ATP in brain tissue of rats after traumatic brain injury were significantly decreased (P <0.05). The mitochondrial ATPase activities of brain tissue in rhEPO group were significantly higher than those in the control group at each time point (P <0.05) at 12, 24 and 48 h after rhEPO treatment. Conclusion Exogenous rhEPO can relieve secondary brain injury after traumatic brain injury by affecting mitochondrial function and thus improve prognosis.