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目的 比较丙氨酰谷氨酰胺二肽 (Ala- Gln)和谷氨酰胺 (Gln)对创伤大鼠静脉营养支持的效果。方法 36只 Wistar雄性大鼠 (2 0 0± 1 0 ) g,随机分为对照、Ala- Gln和 Gln组 ,行创伤和中心静脉插管手术后 ,分别输以不含 Gln、含 1 .8% Ala- Gln或 1 .2 % Gln的等氮、等能量结晶氨基酸注射液 ,喂以无氮饲料 ,实验期为 1 4天。测定氮平衡、血浆氨基酸和蛋白质含量、尾血淋巴细胞转化反应和小肠粘膜核酸、蛋白质含量等指标。结果 Ala- Gln和 Gln组动物术后 8天氮平衡、血浆 Gln的浓度、外周血淋巴细胞转化率和小肠粘膜 DNA、RNA、蛋白质含量均显著高于对照组(P<0 .0 5)。结论 Ala- Gln和 Gln都有助于改善创伤后机体的代谢状况 ,增强免疫功能 ,促进小肠粘膜细胞增殖 ,以维持肠道的完整性 ,Ala- Gln与 Gln具有相同的营养支持效果。
Objective To compare the effects of alanyl-glutamine dipeptide (Ala-Gln) and glutamine (Gln) on parenteral nutritional support in traumatic rats. Methods Thirty-six male Wistar rats (200 ± 10 g) were randomly divided into control, Ala-Gln and Gln groups. After trauma and central venous catheterization, % Ala-Gln or 1.2% Gln of equal nitrogen, such as energy-efficient crystalline amino acid injection, fed with nitrogen-free feed, the experimental period of 14 days. Determination of nitrogen balance, plasma amino acids and protein content, tail blood lymphocyte transformation reaction and intestinal mucosal nucleic acid, protein content and other indicators. Results The nitrogen balance, plasma Gln concentration, peripheral blood lymphocyte transformation rate, DNA, RNA and protein contents in the Ala-Gln and Gln groups were significantly higher than those in the control group on the 8th day after operation (P <0.05). Conclusion Both Ala-Gln and Gln help to improve the metabolic status of post-traumatic organism, enhance the immune function and promote the proliferation of intestinal mucosal cells in order to maintain the integrity of intestinal tract. Ala-Gln and Gln have the same nutritional support effect.